甘肃快3

HUANYINGGUANGLINHANGZHOUWOTENGMOGONGCHENGYOUXIANGONGSIGUANWANG

杭州市
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138-1911-9128
liuhd@dinimedia.com

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甘肃快3

一种重组蛋白质发酵液的分离方法

2016-03-27 22:55:34 杭州沃腾膜工程有限公司 已读
本发明提供了一种利用陶瓷膜对微生物发酵的重组蛋白质发酵液进行固液分离的方法,包括将发酵液在0~30℃的温度下加压到0.1~0.6MPa后进入陶瓷膜过滤系统,发酵液中的微生物和悬浮固体被截留在膜的进料一侧,流出的透过液即为重组蛋白质产物溶液。本发明实现了重组蛋白质发酵液中大分子蛋白质产物与其他固体杂质的有效分离,具有工艺稳定、过滤后透过液澄清度好、便于后续层析处理、易于实现工业化等一系列优点。
权利要求(15)
1.一种利用陶瓷膜对重组蛋白质发酵液进行固液分离的方法,其特征在于,将发酵液在O〜30°C的温度下加压到0.1〜0.6Mpa后进入陶瓷膜过滤系统,发酵液中的菌体细胞和悬浮固体被截留在膜的进料一侧,流出的渗透液即为重组蛋白质产物溶液,其中所述发酵液的PH值为6.0-8.0,所述陶瓷膜的孔径为0.2〜1.2微米。
2.根据权利要求1所述的分离方法,其中的陶瓷膜孔径为0.5〜0.8微米。
3.根据权利要求1或2所述的分离方法,其中发酵液温度为8〜29°C。
4.根据权利要求1或2所述的分离方法,其中发酵液温度为10〜26°C。
5.根据权利要求1或2所述的分离方法,其中重组蛋白质发酵液进入陶瓷膜过滤系统前的压力为0.2〜0.5Mpa。
6.根据权利要求1或2所述的分离方法,其中重组蛋白质发酵液进入陶瓷膜过滤系统前的压力为0.3Mpa。
7.根据权利要求1或2所述的分离方法,其中重组蛋白质发酵液的pH值为6.0。
8.根据权利要求1或2所述的分离方法,其中重组蛋白质发酵液在陶瓷膜过滤系统中的流通量为30〜100升/米2.小时。
9.根据权利要求1或2所述的分离方法,其中重组蛋白质发酵液在陶瓷膜过滤系统中的流通量为75升/米2.小时。
10.根据权利要求1或2所述的分离方法,其中重组蛋白质的分子量为I万〜8万道尔顿。
11.根据权利要求1或2所述的分离方法,其中陶瓷膜过滤系统由一个或多个单元膜系统组成。
12.根据权利要求1或2所述的分离方法,其中重组蛋白质的培养宿主为真核细胞或原核细胞。
13.根据权利要求1或2所述的分离方法,其中重组蛋白质发酵液为微生物发酵的重组蛋白质发酵液。
14.根据权利要求1或2所述的分离方法,其中重组蛋白质发酵液选自下列之一:重组人血白蛋白发酵液、重组人转铁蛋白发酵液。
15.根据权利要求1或2所述的分离方法,将所述重组蛋白质发酵液替换为重组肿瘤坏死因子相关凋亡诱导配体发酵菌体裂解液或重组L-山梨酮脱氢酶发酵菌体裂解液,其中所述发酵菌体裂解液包含微生物菌体碎片、悬浮固体和可溶性重组蛋白质产物,其由发酵液的菌体经超声裂解获得。
说明

甘肃快3 YIZHONGZHONGZUDANBAIZHIFAJIAOYEDEFENLIFANGFA

JISHULINGYU

甘肃快3 [0001] BENFAMINGSHEJIYIZHONGSHENGWUFAJIAOYEFENLIFANGFA,TEBIESHEJIYIZHONGDUIWEISHENGWUFAJIAODEZHONGZUDANBAIZHIFAJIAOYEDEFENLIFANGFA。

BEIJINGJISHU

甘肃快3 [0002] ZAISHENGWUFAJIAOYEFENLILINGYU,ZHONGZUDANBAIZHIFAJIAOYEDEFENLIFANGFAMUQIANZHUYAOBAOKUOLIXINJISHU、BANKUANGGUOLVJISHUHEMOFENSHANGJISHU。

甘肃快3 [0003] LIXINJISHUSHIGENJUKELIZAIZUOYUNSUYUANZHOUYUNDONGSHISHOUDAOYIGEWAIXIANGDELIXINLIDEXINGWEIERFAZHANQILAIDEYIZHONGFENLIJISHU。LIYONGGAIJISHUJINXINGGUYEFENLIZAIDUOGELINGYUGUANGFANYINGYONG,ZHURUFENLICHUHUAXUEFANYINGHOUDECHENDIANWU,TIANRANDESHENGWUDAFENZI、WUJIWU、YOUJIWU,ZAISHENGWUHUAXUEYIJIQITADESHENGWUXUELINGYUCHANGYONGLAISHOUJIXIBAO、XIBAOQIJISHENGWUDAFENZIWUZHI。SUIZHEJISHUDEJINBU,ZHENDUIBUTONGZHUANGTAIDELIAOYE,FAZHANLEBAOKUODIEPIANSHILIXINJIHELIANXULIULIXINJIZAINEIDEDUOZHONGLIXINSHOUDUAN,DANZHEIXIELIXINJISHUCUNZAIZHEQIGUYOUDENANYIKEFUDEQUEDIAN,BAOKUOYUNXINGDONGLICHENGBENGAO、NANYICHULIDALIANGDEGAOHANGULIANGDELIAOYEDENG。

[0004] BANKUANGGUOLVJISHUYONGYUZHONGZUDANBAIZHIYAOWUSHENGCHANZHONGDEGUYEFENLI,QIYOUDIANSHISHEBEITOURUSHAO,CHENGBENDI,QIQUEDIANSHIXUYAOJIAODADERENLITOURU,CUNZAIDALIANGDERENGONGCAOZUO,CONGERNANYISHIXIANHENHAODEPICIZHONGFUXING;WANQUANDEMIFENGKUNNAN,CHANPINSHOULVDI;RANJUNLIAOYECHULIKUNNAN,NANYIBAOZHENGBUTONGLIAOYECHENGQINGDUYIZHI(ZHONGGUOSHENGWUGONGCHENGZAZHI,2004,24 (4):81-85)。

[0005] MOFENLIJISHUZHONG,GENJUSUOYONGMODECAILIAOKEFENWEIGAOFENZIMO、WUJIMOHEYETIMO。CHANGYONGDESHIGAOFENZIMO,QICISHIWUJIMO。GAOFENZIMOCAILIAOYOUXIANWEISULEI、JUXIANANLEI、FANGXIANGZAHUANLEI、JUFENGLEI、JUXITINGLEI、GUIXIANGJIAOLEI、HANFUGAOFENZILEIDENG。GAOFENZIMOYOUDIANSHIZHONGLEIDUO,KEXUANZEXINGDA,JIAGEDI,MOZUJIANJIEGOUJIAOJIANDANDENG;QUEDIANSHIHUAXUEWENDINGXINGHEREWENDINGXINGCHA,YISHOUWEISHENGWUJIANGJIE,YIYAMIDENG。WUJIMODUOYIJINSHU、JINSHUYANGHUAWU、TAOCIDENGZHICHENG,KEYINAIGAOWEN、GAOYA,HUAXUEXINGZHIWENDING,SHOUMINGZHANG,WULIAOTONGLIANGDA,YUNXUSHIYONGKEKEDEQINGXITIAOJIAN;QUEDIANSHIMOCUI,YISUI,SHEBEIFEIGAO(MOFENLIJISHU,HUAXUEGONGYECHUBANSHE,1998)。CHANGYONGDEGAOFENZIMOMOZUJIANJIEGOUXINGSHIZHUYAOYOUPINGBANSHI、GUANSHI、JUANSHIHEZHONGKONGXIANWEISHI4ZHONG。PINGBANSHIMOZUJIANCHANGYONGBANKUANGSHIJIEGOU,QIYOUDIANSHIMODEQINGXIHEGENGHUANRONGYI,LIAOYELIUTONGJIEMIANJIDA,BUYIDUSAI;QUEDIANSHIMIFENGNANDUDA,BUJIANJIAGONGJINGDUYAOQIUGAO,LIAOYELIUCHENGDUAN,WEIDADAOYIDINGNONGSUODUXUDUOCIXUNHUAN,CHANGYONGYUXIAOXINGXUANMOSHIYANZHONG。GUANSHIMOZUJIANJIEGOUYUANLILEISIYUGUANSHIHUANREQI,TADEYOUDIANSHILIAOYELIUSUKEDIAOFANWEIDA,KECHULIXUANFUGUTINONGDUJIAOGAODELIAOYE,QINGXIFANGBIAN;QUEDIANSHIDANWEITIJIMOMIANJIJIAOXIAO,ZAOJIAGAO。JUANSHIMOZUJIANJIEGOUYULUOXUANBANHUANREQILEISI,DANWEITIJIZHONGMOMIANJIDA;DANYALIJIANGDA,MOCENGJIANYIDUSAI,QINGXIKUNNAN。ZHONGKONGXIANWEIMODEWAIJINGWEI0.5〜2mm,KEZAIMOZUJIANNEIZIXINGZHICHENG,MOZUJIANDANWEITIJINEIMOMIANJIDA,YALIJIANGXIAO;DANSHIYIDUSAI,LIAOYEXUYAOYUCHULI(HEBEIKEJIDAXUEXUEBAO,2002,23 (3):21-26)。

[0006] TAOCIMOZAISHENGWUHUAGONGLINGYUDEYINGYONGSHEJIXIBAOTUOCHU、WUJUNSHUISHENGCHANYIJIDIFENZIYOUJIWUDECHENGQINGHESHENGWUMOFANYINGQIDENG。MUQIAN,TAOCIMOZAIZHIYAOCHANYEZHONGDEYINGYONGZHUYAOSHEJIWEISHENGWUZHIYAOZHONGXIAOFENZISHENGWUFAJIAOYEDEGUYEFENLI。CAIYUEMINGDENGGONGKAILELIYONGTAOCIMOFENLIRUSUAN、JIUJINGDENGXIAOFENZISHENGWUFAJIAOYEDEFANGFA(CN1245247C,2006.3.15)。Kim DENG(J CleanerProd, 1997,5(4):263 〜267)YONGJIELIUXIANGDUIFENZIZHILIANGWEI50000DEWUJITAOCIMOCHAOLVFULIUWU,JIELIUDEDAFENZIHEGUTICANZHAJINGLIXINJICHULIHOUYACHENGBINGBO,SHENTOUYEDEZHUYAOCHENGFENSHISHUIHEXIAOFENZIHAIYUANTANG。

[0007] WEISHENGWUFAJIAODEZHONGZUDANBAIZHIFAJIAOYE,LIRU,ZHONGZURENXUEBAIDANBAIFAJIAOYE、ZHONGZURENZHUANTIEDANBAIFAJIAOYEDENGJIAOMUFAJIAOYEYIJIZHONGZUZHONGLIUHUAISIYINZIXIANGGUANDIAOWANGYOUDAOPEITIFAJIAOJUNTILIEJIEYE、ZHONGZUL-SHANLITONGTUOQINGMEIFAJIAOJUNTILIEJIEYEDENGDACHANGGANJUNFAJIAOJUNTILIEJIEYEDENG,GAILEIFAJIAOYEDEDAIFENLIWUZHIJUNWEISHUWANDAOERDUNDEDAFENZIDANBAICHANWU,ERQIEHAIHANYOUJUNTIXIBAOHEXUANFUGUTIDENGZAZHI。DUIYUSHANGSHUZHONGZUDANBAIZHIFAJIAOYEDEFENLIFANGFA,BENLINGYUJISHURENYUANJIXUYIZHONGYOUXIAODEJIEJUEFANGAN。

FAMINGNEIRONG

甘肃快3 [0008] FAMINGRENTONGGUODUONIANYANJIU,LIYONGTAOCIMODUIDUOZHONGZHONGZUDANBAIZHIFAJIAOYEJINXINGLEDALIANGDESHIYAN,CHENGGONGDISHIXIANLEDAFENZIDANBAIZHICHANWUYUJUNTIXIBAO、XUANFUGUTIDENGZAZHIDEYOUXIAOFENLI。

[0009] JUTIDI,BENFAMINGSHEJI:

[0010] (I) YIZHONGLIYONGTAOCIMODUIZHONGZUDANBAIZHIFAJIAOYEJINXINGGUYEFENLIDEFANGFA,QITEZHENGZAIYU,JIANGFAJIAOYEZAIO〜30°CDEWENDUXIAJIAYADAO0.1〜0.6MpaHOUJINRUTAOCIMOGUOLVXITONG,FAJIAOYEZHONGDEJUNTIXIBAOHEXUANFUGUTIBEIJIELIUZAIMODEJINLIAOYICE,LIUCHUDESHENTOUYEJIWEIZHONGZUDANBAIZHICHANWURONGYE。

[0011] (2)GENJUXIANG(I)SUOSHUDEFENLIFANGFA,QIZHONGDETAOCIMOKONGJINGWEI0.01〜2WEIMI,YOUXUANWEI0.2〜1.2WEIMI,TEBIEYOUXUANWEI0.5〜0.8WEIMI。

甘肃快3 [0012] (3)GENJUXIANG⑴HUO⑵SUOSHUDEFENLIFANGFA,QIZHONGFAJIAOYEWENDUWEI8〜29°C,YOUXUANWEI10〜26。。。

甘肃快3 [0013] (4)GENJUXIANG(I)HUO(2)SUOSHUDEFENLIFANGFA,QIZHONGZHONGZUDANBAIZHIFAJIAOYEJINRUTAOCIMOGUOLVXITONGQIANDEYALIWEI0.2〜0.5Mpa,YOUXUANWEI0.3Mpa。

[0014] (5)GENJUXIANG(1)HUO⑵SUOSHUDEFENLIFANGFA,QIZHONGZHONGZUDANBAIZHIFAJIAOYEDEpHZHIWEI5.0〜

8.0,YOUXUANWEI6.0。

[0015] (6)GENJUXIANG(1)HUO(2)SUOSHUDEFENLIFANGFA,QIZHONGZHONGZUDANBAIZHIFAJIAOYEZAITAOCIMOGUOLVXITONGZHONGDELIUTONGLIANGWEI30〜100SHENG/MI2.XIAOSHI,YOUXUANWEI75SHENG/MI2.XIAOSHI。

甘肃快3 [0016] (7)GENJUXIANG(I)HUO(2)SUOSHUDEFENLIFANGFA,QIZHONGZHONGZUDANBAIZHIDEFENZILIANGWEIIWAN〜8WANDAOERDUN。

甘肃快3 [0017] (8)GENJUXIANG(I)HUO(2)SUOSHUDEFENLIFANGFA,QIZHONGTAOCIMOGUOLVXITONGYOUYIGEHUODUOGEDANYUANMOXITONGZUCHENG。

[0018] (9)GENJUXIANG(I)HUO(2)SUOSHUDEFENLIFANGFA,QIZHONGZHONGZUDANBAIZHIDEPEIYANGSUZHUWEIZHENHEXIBAOHUOYUANHEXIBAO。

[0019] (10)GENJUXIANG(I)HUO(2)SUOSHUDEFENLIFANGFA,QIZHONGZHONGZUDANBAIZHIFAJIAOYEWEIWEISHENGWUFAJIAODEZHONGZUDANBAIZHIFAJIAOYE。

[0020] (11)GENJUXIANG⑴HUO⑵SUOSHUDEFENLIFANGFA,QIZHONGZHONGZUDANBAIZHIFAJIAOYEXUANZIXIALIEZHIYI:ZHONGZURENXUEBAIDANBAIFAJIAOYE、ZHONGZURENZHUANTIEDANBAIFAJIAOYE、ZHONGZUZHONGLIUHUAISIYINZIXIANGGUANDIAOWANGYOUDAOPEITIFAJIAOJUNTILIEJIEYEHUOZHONGZUL-SHANLITONGTUOQINGMEIFAJIAOJUNTILIEJIEYE。

甘肃快3 [0021] BENFAMINGSHIYONGDETAOCIMOGUOLVXITONGKEYIYOUDANGEHUODUOGETAOCIMOZUCHENG,QIZHONGDETAOCIMO(GOUZIJIANGSUJIUWUGAOKEGUFENYOUXIANGONGSI)KONGJINGWEI0.01〜2WEIMI,YOUXUANWEI0.2〜1.2WEIMI,TEBIEYOUXUANWEI0.5〜0.8WEIMI。SUOSHUTAOCIMOGUOLVXITONGYEKEYIYOUYIGEHUODUOGEDANYUANMOXITONGZUCHENG,QIZHONGDEDANYUANMOXITONGYOUDUOGETAOCIMOZUCHENG。SHANGSHUTAOCIMOGUOLVXITONGHUODANYUANMOXITONGDEZUCHENGFANGFAWEIBENLINGYUDEGUANCHANGSHOUDUAN,ANZHAOCHANGJIASHUOMINGSHUCAOZUOJIKE。BENLINGYUJISHURENYUANYINGDANGMINGBAI,WEIMANZUDUIZHONGZUDANBAIZHICHANWURONGYEBUTONGCHANLIANGDEYAOQIU,SUOSHUTAOCIMOGUOLVXITONGKEYISHIYONGBUTONGSHULIANGDETAOCIMOHUODANYUANMOXITONG。

[0022] BENFAMINGZHONGDEZHONGZUDANBAIZHIWEILIYONGFENZISHENGWUXUESHOUDUANJIANGMUDEJIYINDAORUYUANHEHUOZHENHEXIBAOZHONG,LIYONGFAJIAOFASHENGCHANMUDEJIYINSUOBIAODADEDANBAIZHI,PEIYANGSUZHUWEIJIAOMUDENGZHENHEXIBAO,YEKEYISHIYUANHEXIBAO。ZUOWEIJULI,BENFAMINGDEZHONGZUDANBAIZHIKEYISHIWEISHENGWUFAJIAODEZHONGZURENXUEBAIDANBAI、ZHONGZURENZHUANTIEDANBAI、ZHONGZUZHONGLIUHUAISIYINZIXIANGGUANDIAOWANGYOUDAOPEITIHUOZHONGZUL-SHANLITONGTUOQINGMEI。

[0023] JINYIBUDI,ZHONGZUDANBAIZHIFAJIAOYEJINRUTAOCIMOGUOLVXITONGQIANDEYALIWEI0.1〜0.6Mpa,YOUXUANWEI0.2〜0.5Mpa,TEBIEYOUXUANWEI0.3Mpa。FAJIAOYEWENDUWEIO〜30°C,YOUXUANWEI8〜29°C,TEBIEYOUXUANWEI10〜26°C,GENJUDANBAIZHIDEXINGZHI,WENDUKONGZHIKEYISHIHENGWEN,YEKEYISHIBIANWEN。FAJIAOYEpHZHIWEI5.0〜8.0,YOUXUANWEI6.0。FAJIAOYEZAITAOCIMOGUOLVXITONGZHONGDELIUTONGLIANGWEI30〜100SHENG/MI2 •XIAOSHI,YOUXUANWEI75SHENG/MI2.XIAOSHI。

[0024] YUCAIYONGLIANXULIULIXINJIHUOZHONGKONGXIANWEIMODENGCHUANTONGFENLIGONGYIXIANGBI,BENFAMINGDEZHONGZUDANBAIZHIFAJIAOYEDEFENLIFANGFA,CHULEJUYOUTAOCIMODEHUAXUEWENDINGXINGHAO(NAISUAN、NAIJIAN、NAIYANGHUA、NAIYOUJIRONGJI),NAIGAOWEN,JIXIEQIANGDUDA,NAIMOXINGHAO,FENLIJINGDUGAO(KEDANAMIJIGUOLV)YIJIYIQINGXIDENGYOUDIANZHIWAI,HAIJUYOUGONGYIWENDING、CHENGBENDILIAN、FENLIJINGDUJIGAO,SUOXURENLISHAO,NENGGOUYUHOUXUDECENGXICHULIDADAOGENGHAODEXIANJIE,FEICHANGYIYUGONGYEHUADENGYOUDIAN。

[0025] JUTIDI,BENFAMINGTONGGUOKONGZHITAOCIMOGUOLVXITONGZHONGZHONGZUDANBAIZHIFAJIAOYEDEWENDU、YALI、LIUTONGLIANGHETAOCIMOKONGJINGDENGCANSHU,KEYISHIXIANLIAOYEDEGAOCHENGQINGDUFENLI,JIANGDICENGXIDESHANGZHUYALI,TIGAOCENGXIZHUCHULILIUSU,SHIXIANLEYUHOUXUCENGXICHULIDEGENGHAOXIANJIE,TIGAOLEGONGZUOXIAOLV。LINGWAI,BENFAMINGTIGONGDEFANGFAZAIDAGUIMOSHENGCHANZHONGKEYICAIYONGXIAOGUIMOSHIYANZHONGSUOQUEDINGDEGONGYICANSHU,RUTAOCIMOLIUDAOZHANGDUHEGUANJING,ZHIXUSHULIANGSHANGDEXIANXINGZENGJIA,FEICHANGYIYUGONGYEHUAFANGDA,JUYOUJIGAODECHANYEHUAYINGYONGJIAZHI。

FUTUSHUOMING

甘肃快3 [0026] TU1WEITAOCIMOGUOLVXITONGLIULUSHIYITU。

甘肃快3 [0027] TU2WEIZHONGZURENXUEBAIDANBAIFAJIAOYESDS-PAGEDIANYONGTU,QIZHONGYONGDAOAWEILIXINHOUSHANGQING,YONGDAOBWEIJINGGUOTAOCIMOGUOLVHOUSHANGQING,YONGDAOCWEIDANBAIZHIFENZILIANGBIAOZHUNCONGDADAOXIAOWEI(97400,66200,43000,31000)。

[0028] TU3WEIZHONGZUZHONGLIUHUAISIYINZIXIANGGUANDIAOWANGYOUDAOPEITIFAJIAOYESDS-PAGEDIANYONGTU,QIZHONGYONGDAOAWEILIXINHOUSHANGQING,YONGDAOBWEIJINGGUOTAOCIMOGUOLVHOUSHANGQING,YONGDAOCWEIDANBAIZHIFENZILIANGBIAOZHUNCONGDADAOXIAOWEI(97400,66200,43000,31000,20100,14400)。

甘肃快3 [0029] TU4WEIZHONGZUL-SHANLITONGTUOQINGMEIFAJIAOYESDS-PAGEDIANYONGTU,QIZHONGYONGDAOaWEILIXINHOUSHANGQINGPBAD/SND,YONGDAObWEIDANBAIZHIFENZILIANGBIAOZHUNCONGDADAOXIAOWEI(94000,67000,43000,30000)。

[0030] TU5WEIZHONGZURENXUEBAIDANBAIFAJIAOYEZAITAOCIMOGUOLVXITONGZHONGDETONGLIANGSHUAIJIANQUXIAN。

[0031] TU6WEIZHONGZU -SHANLITONGTUOQINGMEIFAJIAOJUNTILIEJIEYEZAITAOCIMOGUOLVXITONGZHONGDETONGLIANGSHUAIJIANQUXIAN。

甘肃快3 JUTISHISHIFANGSHI

[0032] XIASHUSHISHILIJUTICHANSHUBENFAMINGZHONGZUDANBAIZHIFAJIAOYEDEFENLIFANGFA,JINWEIBENLINGYUJISHURENYUANGENGHAODILIJIEBENFAMING,BUYINGJIESHIWEIDUIBENFAMINGDEXIANZHI。[0033] SHISHILI1LIYONGTAOCIMOGUOLVZHONGZURENXUEBAIDANBAIFAJIAOYE

[0034] YINGYONGZIYOUZHUANLISHENQINGJISHU(CN1854301AHECN1854306A)GOUJIANDEJIYINGONGCHENGJUNJISHENGCHANFANGFAHUODEZHONGZURENXUEBAIDANBAIFAJIAOYE,QIJUNTINONGDUWEI40 %,DANBAIZHINONGDU16g/L,DANBAIZHIFENZILIANGWEI66KD。

甘肃快3 [0035] ZAI24_30°CXIA,JIANGSHANGSHUFAJIAOYE(pHZHIWEI6.0)JIAYADAO0.3MpaHOUZHIJIEJINRUTAOCIMOGUOLVXITONG(TAOCIMOKONGJINGWEI0.5WEIMI)。FAJIAOYEZHONGDEJIAOMUXIBAOHEXUANFUGUTIDENGBEIJIELIUZAIMODEJINLIAOYICE,TONGGUOHUILIUTONGLUHUIDAOYUANLIAOGUAN,ERSHENTOUYEJIWEIZHONGZUDANBAIZHICHANWURONGYE。DANGSHIZHONGDADAO60% (JINLIAO50L,SHENTOUYEWEI21L)SHI,KAISHITONGGUOQINGXITONGLUJINXINGLIUJIASHUICAOZUO,GONGLIUJIASHUI20L,DANGJIANCEDAOHUILIUDELVZHAYEZHONGSHIZHONG70%,LVZHAYESHANGQINGDANBAIZHINONGDUDIYUlg/LSHITINGZHISHOUJI,GONGHUODESHANGQINGYE45L。FAJIAOYEZAITAOCIMOGUOLVXITONGZHONGDETONGLIANGSHUAIJIANQUXIANJIANFUTU5。FAJIAOYEDIANYONGJIEGUOJIANTU2。JUTISHIYANSHU

JUJIANXIABIAO。

甘肃快3 [0036]

甘肃快3 [0037] SHISHILI2LIYONGTAOCIMOGUOLVZHONGZURENZHUANTIEDANBAIFAJIAOYE

甘肃快3 [0038] YINGYONGZIYOUJISHUGOUJIANDEBIAODARENZHUANTIEDANBAIDENIANGJIUJIAOMUGONGCHENGJUN,JINGFAJIAOSUOHUODEFAJIAOYEDEJUNTINONGDUWEI20%,DANBAIZHINONGDU0.lg/L,DANBAIZHIFENZILIANGWEI75KD。ZAI24-26°CXIA,JIANGSHANGSHUFAJIAOYE(pHZHIWEI6.0)JIAYADAO0.2MpaHOUZHIJIEJINRUTAOCIMOGUOLVXITONG(TAOCIMOKONGJINGWEI1.2WEIMI),FAJIAOYEZHONGDEJIAOMUXIBAO、XUANFUGUTIDENGBEIJIELIUZAIMODEJINLIAOYICE,TONGGUOHUILIUTONGLUHUIDAOYUANLIAOGUAN,ERSHENTOUYEJIWEIZHONGZUDANBAIZHICHANWURONGYE。DANGSHIZHONGDADAO60% (JINLIAO30L,SHENTOUYEWEI15L)SHI,KAISHITONGGUOQINGXITONGLUJINXINGLIUJIASHUICAOZUO,GONGLIUJIASHUI12L,DANGJIANCEDAOHUILIUDELVZHAYEZHONGSHIZHONG70%,LVZHAYESHANGQINGDANBAIZHINONGDUDIYU0.005g/LSHITINGZHISHOUJI,HUODESHANGQINGYE15L,LEIJIHUODESHANGQINGYE30L。JUTISHIYANSHUJUJIANXIABIAO。[0039]

[0040] SHISHILI3LIYONGTAOCIMOGUOLVZHONGZUZHONGLIUHUAISIYINZIXIANGGUANDIAOWANGYOUDAOPEITIFAJIAOJUNTILIEJIEYE

[0041] YINGYONGZIYOUJISHUGOUJIANDEBIAODAZHONGZUZHONGLIUHUAISIYINZIXIANGGUANDIAOWANGYOUDAOPEITIDEDACHANGGANJUNGONGCHENGJUN,JINGFAJIAOSUOHUODEFAJIAOYEDEJUNTINONGDUWEI10%,MUBIAODANBAIZHINONGDUWEI5g/L,DANBAIZHIFENZILIANGWEI18KD。ZAI4°CXIACHAOSHENGLIEJIEJUNTI,JIANGSHANGSHULIEJIEYE(pHZHIWEI7.0,GUXINGWUNONGDUWEI7%,LIXINCHENGZHONGFACEDING)ZAI10-15°CXIAJIAYADAO0.5MpaHOUZHIJIEJINRUTAOCIMOGUOLVXITONG(TAOCIMOKONGJINGWEI0.2WEIMI),LIEJIEYEZHONGDEWEISHENGWUJUNTISUIPIAN、XUANFUGUTIDENGBEIJIELIUZAIMODEJINLIAOYICE,TONGGUOHUILIUTONGLUHUIDAOYUANLIAOGUAN,ERSHENTOUYEJIWEIZHONGZUDANBAIZHICHANWURONGYE。DANGSHIZHONGDADAO50% (JINLIAO30L,SHENTOUYEWEI25L)SHI,KAISHITONGGUOQINGXITONGLUJINXINGLIUJIASHUICAOZUO,GONGLIUJIASHUI10L,DANGJIANCEDAOHUILIUDELVZHAYEZHONGSHIZHONG70%,LVZHAYESHANGQINGDANBAIZHINONGDUDIYU0.lg/LSHITINGZHISHOUJI,HUODESHANGQINGYE11L,LEIJIHUODESHANGQINGYE36L。FAJIAOYE

甘肃快3 DIANYONGJIEGUORUTU3SUOSHI。JUTISHIYANSHUJUJIANXIABIAO。

[0042]

甘肃快3 PINGJUNTONGLIANG43L/m2.h ZONGSHOULV:97%,QIZHONGYILIXINSHOULVWEI100%

[0044] SHISHILI4LIYONGTAOCIMOGUOLVZHONGZUL-SHANLITONGTUOQINGMEIFAJIAOJUNTILIEJIEYE

[0045] LIYONGZIYOUZHUANLIJISHU(ZL200310116831.7)GOUJIANDEJIYINGONGCHENGJUNJINXINGFAJIAO,SUOHUODEDACHANGGANJUNFAJIAOYEDEJUNTINONGDUWEI4%,DANBAIZHINONGDU2g/L,DANBAIZHIFENZILIANGWEI45KD,

甘肃快3 [0046] JIANGFAJIAOYEJINXINGSHOUJIHOU,5000r/minXIALIXIN5minSHOUJIJUNTI,YONGpH8.0DENa2HPO4-NaH2PO4HUANCHONGYEXIDI3CI,ANZHAO1:10DEBILIJIANGLIXINHOUDEJUNTIRONGJIEYUCHAOSHENGHUANCHONGYE(YULENGDESHENGLIYANSHUIHUOpH8.0DETris-HCl)ZHONG,500w,10s/10sPOSUI20FENZHONG。ZAI4°CXIA,JIANGSHANGSHUCHAOSHENGLIEJIEYE(pHZHIWEI8.0)JIAYADAO0.3MpaHOUZHIJIEJINRUTAOCIMOGUOLVXITONG(TAOCIMOKONGJINGWEI0.5WEIMI),LIEJIEYEZHONGDEXUANFUGUTI(BAOKUOXIBAOSUIPIANHEBAOHANTI)BEIJIELIUZAIMODEJINLIAOYICE,TONGGUOHUILIUTONGLUHUIDAOYUANLIAOGUAN,ERSHENTOUYEJIWEIZHONGZUDANBAIZHICHANWURONGYE。DANGSHIZHONGDADAO30%SHI,KAISHITONGGUOQINGXITONGLUJINXINGLIUJIASHUICAOZUO,GONGLIUJIASHUI25L,DANGJIANCEDAOHUILIUDELVZHAYESHANGQINGDANBAIZHINONGDUDIYU0.05g/LSHITINGZHISHOUJI,GONGHUODESHANGQINGYE61L。FAJIAOYEDIANYONGJIEGUORUTU4SUOSHI。LIEJIEYEZAITAOCIMOGUOLVXITONGZHONGDETONGLIANGSHUAIJIANQUXIANJIANFUTU6。JUTISHIYANSHUJUJIANXIABIAO。

[0047]

甘肃快3 [0048] SHISHILI5LIYONGTAOCIMOGUOLV,SHIXIANGENGHAODECENGXIXIANJIE

甘肃快3 [0049] JIANGZHONGZURENXUEBAIDANBAIFAJIAOYEFENBIEJINXINGLIXIN(15°C,5000ZHUAN/FEN,30FENZHONG)HETAOCIMOGUOLV(SHISHILI1)CHULI,LIXINHOUSHANGQINGZHONGYOUWEILIANGDEGUTIXUANFU,TAOCIMOTOUGUOYEDECHENGQINGDUMINGXIANJIAOGAO;JIANGSHANGSHULIXINHOUSHANGQINGHETAOCIMOTOUGUOYEYITONGYANGDECHUNHUAGONGYI(SHENQINGRENZIYOUZHUANLIJISHUEP1504031B1,CENGXIZHUCAIYONGBPG140)CHULI,TAOCIMOTOUGUOYEDECENGXISHANGZHUYALIJIAODI(TONGYANGXIANXINGLIUSUXIA,LIXINHOUSHANGQINGDESHANGZHUYALIWEI0.15MPa,TAOCIMOTOUGUOYEDESHANGZHUYALIWEI0.09Mpa)。TONGYANGDESHANGZHUYALITIAOJIANXIA,TAOCIMOTOUGUOYEDESHANGZHULIUSUJIAOGAO(LIXINHOUSHANGQINGDESHANGZHULIUSUWEI80cm/h,TAOCIMOTOUGUOYEDESHANGZHULIUSU120cm/h),SHANGYANGSHIJIANJIAOLIXINHOUSHANGQINGSUODUANLE 50%,TIGAOLECENGXIGONGZUOXIAOLV,CONGERSHIXIANLEGENGHAODEGONGYIXIANJIE。

甘肃快3 [0050] SHISHILI6TAOCIMOGUOLVDEGONGYEHUAFANGDASHIYAN

甘肃快3 [0051] LIYONGZHONGZURENXUEBAIDANBAIFAJIAOYEJINXINGSANGEJIEDUANDESHIYAN。DIYIJIEDUANLIYONGDANGETAOCIMOWANCHENG,CAOZUODEJUTICANSHUWEI:TAOCIMOKONGJINGWEI0.5WEIMI,FAJIAOYEXUNHUANGUOLVXITONGWENDUWEICHI25V’BENGRUYALI0.3MPa。DANGSHIZHONGDADAO60%SHI,KAISHITONGGUOQINGXIYETONGLUJINXINGLIUJIASHUICAOZUO,LIUJIASHUITIJIWEICHUSHIFAJIAOYETIJIDE0.5BEI,DANGJIANCEDAOHUILIUDELVZHAYEZHONGSHIZHONG70%,LVZHAYESHANGQINGDANBAIZHINONGDUDIYUlg/LSHITINGZHISHOUJI。DIERJIEDUANHEDISANJIEDUANFENBIELIYONGXITONGHUAJICHENG16GEHE288GETAOCIMO(MOMIANJIFENBIEWEI3.5PINGFANGMIHE63PINGFANGMI)JINXINGZHONGSHIHESHENGCHANSHUIPINGDESHIYAN,SHIYANCANSHUYUDIYIJIEDUANXIANGTONG。

[0052] DUIBISANGEJIEDUANSUOSHOUHUODEFENLIHOUDEFAJIAOYEZHILIANG,CHENGQINGDUXIANGDANG,CENGXISHANGZHUYALIWEICHIZAI

甘肃快3 0.09MPa,SHANGZHULIU SUJUNDADAO120cm/h。

 

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